PSeq™ Technology

PSeq — A New Category Built Around Resolution

Sequencing advanced through foundation, scale, and read length. PSeq adds molecule-level isoform resolution. By preserving molecular identity through an NGS workflow, it allows reads from the same captured template to be grouped before transcript reconstruction.

Not another sequencer.A new architecture for molecule-resolved RNA measurement.

Foundation

Sanger
Sequencing

First-generation sequencing

Scale

Next Generation
Sequencing

Sequencing at scale

Length

Third Generation
Sequencing

Long-read sequencing

Resolution
Phased SequencingPSeq

Molecular identity • NGS • isoform assembly

How PSeq Technology Works

Three connected stages transform individual RNA molecules into complete, analysis-ready sequence data.

01Identify

Tag every molecule

Each RNA molecule is reverse-transcribed using one of 4.3 trillion multifunctional tagging reagents, uniquely labeling individual molecules.

4.3 trillion-tag molecular identity space
02Construct

Build within each molecule

Tagged molecules are converted into sequencing-ready DNA libraries using reactions confined to intramolecular rearrangements.

Sequencing-ready DNA libraries
03Resolve

Process data end to end

Standard FASTA files from the sequencer are processed end-to-end by the automated PSeq data pipeline.

Complete, analysis-ready output

From Sample to Isoform-Resolved Data

PSeq moves from molecular preparation through automated processing to structured, molecule-level output—within the NGS workflow already in use.

Apply

PSeq Chemistry

Four molecular preparation steps preserve the identity and structure of each RNA molecule.

  1. Molecular taggingReverse transcription with individual barcodes and strand discriminators.
  2. Isothermal amplificationPCR-free amplification.
  3. Intermolecular UMI redistributionSMIDs are relocated to the start of Read 1 in standard paired-end NGS libraries.
  4. Companion toolsRetrieve individual, barcode-identified molecules discovered by sequencing.
Execute

PSeq Software

An automated, molecule-aware pipeline carries sequencing data through preprocessing, quality control, molecular assignment, transcript reconstruction, and structured output.

  • Molecule-aware processingPreprocessing and quality control prepare reads for molecule-specific assignment, which is evaluated before transcript assembly.
  • Molecule-constrained reconstructionWith sufficient depth and coverage, reads assigned to each captured molecular template support a more direct view of individual isoforms than inference from pooled fragments.
  • Interoperable outputsStructured isoform-level results can connect with biological databases and established bioinformatics, visualization, ML, and AI tools.
Deliver

Isoform‑Resolved Insights

Individual-molecule data, structured for analysis and discovery.

  • Isoform architectureConnected exon and UTR structure within individual RNA molecules.
  • Protein-coding potentialOpen reading frames and alternative initiation sites.
  • PolyadenylationAlternative cleavage and polyadenylation sites.
  • ML-ready outputsStructured, molecule-level features designed as inputs to downstream ML models.