PSeq — A New Category of Sequencing Technology
(NGS)
How PSeq Technology Works
Single-molecule tagging
Each RNA molecule is reverse-transcribed using one of 4.3 trillion multifunctional tagging reagents, uniquely labeling individual molecules.
Intramolecular library construction
Tagged molecules are converted into sequencing-ready DNA libraries using reactions confined to intramolecular rearrangements.
Automated data processing
Standard FASTA files from the sequencer are processed end-to-end by the automated PSeq data pipeline.
PSeq long-read sequencing runs on unmodified NGS sequencers now widely in service, eliminating the need to purchase specialized Third Generation instruments and robotics, or hire highly specialized staff training.
PSeq Workflow
Phaeno PSeq phased long-read technology is easy to use and easily fits into existing workflows.
Sample
Chemistry
System
Software
Stack
Resolved
Insights
apply: PSEQ Chemistry
Our sample preparation protocol consists of five steps.
Molecular tagging: reverse transcription molecular tagging with individual barcodes and strand discriminators.
Isothermal amplification: PCR-free amplification.
Intermolecular UMI (tag) redistribution: SMIDs are relocated intramolecularly to the start of Read 1 in standard paired-end NGS libraries.
Companion tools: retrieval of individual, barcode identified molecules, discovered by sequencing.
execute: PSeq Software Stack
Phaeno's fully-automated pipeline enables direct, rapid data assembly.
Fully automated compute; data polishing unnecessary.- No use of priors or statistical algorithms.
- Includes in silico tools for generating AI/ML training sets.
deliver: Isoform Resolved Insights
Detailed data at the individual molecule level. How you want it.
- Data consolidation for low-cost transmission, and optimized for ML.
- ORF-constitutive and alternative protein-coding exons
- Alternative translation initiation sites
- Alternative polyadenylation (chain termination) sites
- RNA trafficking “zip” codes
- And much more...