Abstract
PSeq is a research-use RNA-sequencing platform that brings whole-molecule resolution and end-to-end phasing to standard short-read next-generation sequencing (NGS). During reverse transcription, a source-molecule tagging reagent labels each RNA sequence, and a library strategy preserves the tag marker across random fragments. Paired-end sequencing runs on unmodified instruments, while an automated pipeline groups reads by Source Molecule Identifier (SMID), identifies the source gene, assembles a consensus sequence, and retains supporting records in a run-specific SQL database.
This paper presents the platform’s core problem, architecture, and operating model. It omits operational reagent recipes and detailed validation results, which are covered in companion papers.
Key topics
- Source-molecule identity established before library fragmentation
- SMID-based read grouping and end-to-end transcript reconstruction
- Coupled molecular tagging, automated assembly, and run-specific SQL records
- Deployment on standard paired-end short-read NGS infrastructure
Contents
- The short-read linkage problem
- PSeq Clear-Signal Architecture and design principles
- End-to-end operating model
- Three coupled platform components
- Molecule-level analysis and evidence traceability
- Deployment model, deliverables, and current boundaries